A Novel Sample Preparation Device for Improved Phospholipid Removal in Bioanalytical Assays

int roduCt ion Matrix effects are residual components of biological matrix that alter mass spectrometry response, decrease method robustness, add to method variability, and lead to poor limits of detection/quantification. A major source of matrix effects in plasma-based bioanalytical assays is residual phospholipids. If not removed from the sample, phospholipids can build up on analytical columns, elute at unpredictable times in subsequent injections, and negatively impact assay robustness in a number of different ways. Over the past few years, it has become commonplace to monitor phospholipids during method development and to implement strategies to remove them from plasma samples. Sample preparation strategies for phospholipid (PL) removal include the use of specific PL removal plates, liquid-liquid extraction (LLE), and solid-phase extraction (SPE.) During the method development process, decisions are made regarding the choice of a “fit-for-purpose” sample prep technique. OstroTM Sample Preparation Products are designed to provide a novel solution for the rapid and simple cleanup of phospholipids in biological samples prior to LC/MS/MS analysis. Using a convenient 96-well format, in-well protein precipitation is performed with a single pass through method which provides consistent, high quality results, including significant removal of phospholipids for cleaner extracts, optimal recovery for diverse analytes, and increased reproducibility for consistent, robust methods.